Intranasal Peptide Spray Calculator
For an intranasal spray, the number that matters is micrograms per pump. It depends on the total liquid you mix — bacteriostatic water plus saline — and how much each pump delivers.
The formula
Total volume = water + saline
Concentration = peptide ÷ total volume
Per pump = concentration × pump volume
Concentration = peptide ÷ total volume
Per pump = concentration × pump volume
Worked example
Example
Peptide in vial10 mg
Bacteriostatic water + saline1 mL + 1 mL = 2 mL
Concentration5,000 mcg/mL
Pump volume0.1 mL
Per pump500 mcg
That 2 mL bottle would deliver about 20 pumps in total.
How to use the calculator
- Enter the peptide amount in the vial.
- Enter your bacteriostatic water and saline volumes separately.
- Set the volume per pump (defaults to 0.1 mL).
- Optionally enter a target dose to see how many whole pumps reach it.
Frequently asked questions
How do I calculate mcg per pump for a nasal spray?
Add your bacteriostatic water and saline together for the total volume, divide the peptide amount by that total to get concentration in mcg/mL, then multiply by the volume the pump delivers per actuation — commonly about 0.1 mL. The result is the micrograms delivered per pump.
Why mix both bacteriostatic water and saline?
The bacteriostatic water typically dissolves and preserves the peptide, while saline brings the solution closer to isotonic, which is gentler on the nasal lining. Both liquids count toward the total volume, so both affect the final concentration and the dose per pump.
How much does one nasal pump deliver?
Metered nasal pumps are commonly around 0.1 mL (100 microlitres) per actuation, but it varies by pump. The calculator defaults to 0.1 mL and lets you change it to match your hardware's stated delivery.
How many pumps will my bottle give?
Divide the total liquid volume by the per-pump volume. Real bottles lose a little to priming and dip-tube dead space, so plan for slightly fewer usable pumps than the theoretical number.