Enter your vial strength and the water you're adding — get concentration, the exact draw on an insulin syringe, and how many doses are in the vial. No guesswork.
Concentration = peptide ÷ water. A 5 mg vial in 2 mL of water gives 2.5 mg/mL (2,500 mcg/mL).
Draw volume = dose ÷ concentration. A 250 mcg dose ÷ 2,500 mcg/mL = 0.1 mL.
Insulin units = volume × 100, because a U-100 syringe marks 100 units per millilitre. So 0.1 mL = 10 units... here it's 12.5 because the default dose is 250 mcg from a 2,500 mcg/mL mix — the live number above always reflects your inputs.
Less water → higher concentration → fewer units per dose. More water makes small doses easier to measure accurately.
Total volume = bacteriostatic water + saline. Both liquids count toward how dilute the spray is — the water typically dissolves and preserves the peptide, the saline brings it closer to an isotonic, nose-friendly mix.
Concentration = peptide ÷ total volume. A 10 mg vial in 2 mL total = 5,000 mcg/mL.
Per pump = concentration × pump volume. A metered nasal pump usually delivers about 0.1 mL, so 5,000 mcg/mL × 0.1 mL = 500 mcg per pump.
Pumps per bottle = total volume ÷ pump volume. Real bottles lose a little to priming and the dip-tube dead space, so expect a few fewer usable pumps than the theoretical count.
Each half-life cuts the amount in half. After n half-lives, (½)ⁿ of the dose remains — so 1 half-life leaves 50%, two leaves 25%, three leaves 12.5%, and so on.
Single dose shows that decay curve and the exact amount left at the time you enter.
Repeated dosing stacks each new dose on top of what hasn't cleared yet. Levels keep climbing until clearance balances intake — that plateau is steady state, reached after about 5 half-lives. The accumulation factor tells you how much higher the steady-state peak sits versus a single dose.