mixmy pep.
Reconstitution, dosing & half-life math — done right, in seconds.

Peptide Mixing Chart

A lookup table for the two numbers that come up every time you mix a vial: the concentration you end up with, and how many units that makes you draw. No arithmetic required — find your vial size, find your water volume, read the cell.

Illustrative reference only — nothing here recommends a dose or a mixing volume.

Concentration by vial size and water volume

Concentration is simply the peptide in the vial divided by the water you add, so the same vial can be any strength you like. Adding less water makes a stronger mix and a smaller draw; adding more makes a weaker mix and a larger, easier-to-read draw.

Concentration (mg/mL) for a vial reconstituted with a given volume of bacteriostatic water
Vial1 mL2 mL3 mL5 mL
2 mg210.6670.4
5 mg52.51.6671
10 mg1053.3332
15 mg157.553
20 mg20106.6674
30 mg3015106
50 mg502516.66710

To read this as mcg/mL instead, multiply by 1,000 — a 2.5 mg/mL mix is 2,500 mcg/mL.

Units to draw, by mix strength and dose

Once you know the concentration, the draw follows: divide your dose by the concentration to get millilitres, then multiply by 100, because a U-100 syringe is marked 100 units per millilitre.

Units to draw on a U-100 insulin syringe, by mix strength and dose
Mix100 mcg250 mcg500 mcg1,000 mcg2,000 mcg
1 mg/mL10 u25 u50 u100 u>100 u
2 mg/mL5 u12.5 u25 u50 u100 u
2.5 mg/mL4 u10 u20 u40 u80 u
5 mg/mL2 u5 u10 u20 u40 u
10 mg/mL1 u2.5 u5 u10 u20 u

Draws under about 2 units are hard to measure accurately; anything over 100 units won't fit in a 1 mL syringe in one go. Both are signs to change the water volume rather than the dose.

Open the live calculator

The chart covers the common combinations. For any vial, water volume and dose — including a target draw worked backwards into a water volume — use the reconstitution calculator.

Frequently asked questions

How do I use a peptide mixing chart?
Find the row for the amount of peptide in your vial and the column for the volume of bacteriostatic water you are adding. The cell is the concentration in mg per mL. Then use the second table: find that concentration and your intended dose to read the units to draw on a U-100 insulin syringe.
Does adding more water reduce the amount of peptide?
No. The vial contains a fixed amount of peptide regardless of how much water you add. Water only sets the concentration, which changes how large each draw is. A 10 mg vial holds 10 mg whether it is mixed with 1 mL or 5 mL — you simply draw five times the volume for the same dose in the weaker mix.
What concentration should I mix to?
That is a decision this site does not make. Mechanically, a concentration that puts your intended draw somewhere in the readable middle of the syringe is easier to measure accurately than one that lands at one or two units. The chart shows what each combination produces so you can see the trade-off.
Why are some cells marked over 100 units?
A 1 mL U-100 insulin syringe holds 100 units. If a dose at that concentration needs more than that, it cannot be drawn in one go — it would need more than one draw, a larger syringe, or a stronger mix made with less water.